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熊治廷、博士生刘荣相的论文在INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 刊出
发布时间:2021-06-30 17:45:07     发布者:易真     浏览次数:

标题: Comparison and Characterization of a Cell Wall Invertase Promoter from Cu-Tolerant and Non-Tolerant Populations of Elsholtzia haichowensis

作者: Liu, RX (Liu, Rongxiang); Zhao, J (Zhao, Jing); Xu, ZR (Xu, Zhongrui); Xiong, ZT (Xiong, Zhiting)

来源出版物: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES : 22 : 10 文献号: 5299 DOI: 10.3390/ijms22105299 出版年: MAY 2021

摘要: Cell wall invertase (CWIN) activity and the expression of the corresponding gene were previously observed to be significantly elevated in a Cu-tolerant population of Elsholtzia haichowensis relative to a non-tolerant population under copper stress. To understand the differences in CWIN gene regulation between the two populations, their CWIN promoter beta-glucuronidase (GUS) reporter vectors were constructed. GUS activity was measured in transgenic Arabidopsis in response to copper, sugar, and phytohormone treatments. Under the copper treatment, only the activity of the CWIN promoter from the Cu-tolerant population was slightly increased. Glucose and fructose significantly induced the activity of CWIN promoters from both populations. Among the phytohormone treatments, only salicylic acid induced significantly higher (p < 0.05) activity of the Cu-tolerant CWIN promoter relative to the non-tolerant promoters. Analysis of 5 '-deletion constructs revealed that a 270-bp promoter fragment was required for SA induction of the promoter from the Cu-tolerant population. Comparison of this region in the two CWIN promoters revealed that it had 10 mutation sites and contained CAAT-box and W-box cis-elements in the Cu-tolerant promoter only. This work provides insights into the regulatory role of SA in CWIN gene expression and offers an explanation for differences in CWIN expression between E. haichowensis populations.

入藏号: WOS:000661993600001

语言: English

文献类型: Article

作者关键词: cell wall invertase; Elsholtzia haichowensis; promoter; populations; salicylic acid; copper; sugar; phytohormone; cis elements

地址: [Liu, Rongxiang; Zhao, Jing; Xu, Zhongrui; Xiong, Zhiting] Wuhan Univ, Hubei Biomass Resource Chem & Environm Biotechnol, Sch Resource & Environm Sci, Wuhan 430079, Peoples R China.

[Liu, Rongxiang] Qiandongnan Vocat & Tech Coll Nationalities, Kaili 556000, Peoples R China.

通讯作者地址: Xiong, ZT (通讯作者)Wuhan Univ, Hubei Biomass Resource Chem & Environm Biotechnol, Sch Resource & Environm Sci, Wuhan 430079, Peoples R China.

电子邮件地址: liurongxiang@whu.edu.cn; zhaojing@whu.edu.cn; ztxiong@whu.edu.cn; xuzhongrui@njau.edu.cn

影响因子:4.556


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